Beilstein J. Org. Chem.2013,9, 966–973, doi:10.3762/bjoc.9.111
, click ligation of a fluorescent dye, and gel electrophoresis revealed specific labeling of a single 24 kDa band that could be blocked with an active competitor. Future work will focus on identifying the labeled protein(s).
Keywords: CCG-1423; click ligation; photoaffinity labeling; Rhopathway
inhibitor; tag-free photoprobe; target identification; Findings
Serum-induced signaling through Rho leads to gene-transcriptional effects, which are mediated by serum response factor (SRF), a MADS box transcription factor that binds to the serum response element (SRE) in the promoters of various immediate
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Graphical Abstract
Figure 1:
Structures of lead Rho/MKL1/SRF inhibitor 1 and conformationally restricted analogue 2.